Forward Gianni Evangelisti and defenseman Shawn Baird were suspended one game by the American Collegiate Hockey Association on Thursday.
According to an email from coach Sean Hogan, the two players were suspended for their involvement in an altercation in Saturday's game against Pittsburgh at Bird Arena.
Evangelisti and Baird will serve their suspensions Friday night when Ohio plays Robert Morris-Illinois in Bensenville, Illinois.
Pitt forwards Vincent Spino and Patrick Smith were also suspended, according to Hogan.
Evangelisti leads Ohio with 38 points, while Baird has tallied seven.
Tom Pokorney was assessed Ohio's first suspension of the year Jan. 7 after being called for a head contact penalty against Lindenwood on Jan. 6. The Bobcats have also dealt with a short roster this season due to injuries and transfers.